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  Indian J Med Microbiol
 

Figure 4: Schematic representation of isolation, enrichment, and culture methods of SSCs. The testicular tissues is digested using the enzyme. Then, MACS, differential plating, Percoll gradient, or FACS is used for the enrichment of SSCs. In two-dimensional cell culture system, SSCs are cultured on feeder cells. Soft agar and MC are the most commonly used media in the three-dimensional cell culture system. SSC: spermatogonial stem cell; MACS: magnetic-activated cell sorting; FACS: fluorescence-activated cell sorting; MC: methylcellulose.

Figure 4: Schematic representation of isolation, enrichment, and culture methods of SSCs. The testicular tissues is digested using the enzyme. Then, MACS, differential plating, Percoll gradient, or FACS is used for the enrichment of SSCs. In two-dimensional cell culture system, SSCs are cultured on feeder cells. Soft agar and MC are the most commonly used media in the three-dimensional cell culture system. SSC: spermatogonial stem cell; MACS: magnetic-activated cell sorting; FACS: fluorescence-activated cell sorting; MC: methylcellulose.