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  Indian J Med Microbiol
 

Figure 3: Effect of knockdown of endogenous miR-3691-3p target genes on the behavior of PCa cells. (a) Of the 200 target genes identified from three bioinformatic algorithms (DIANA-MICROT, Target Scan, and MIRDB), 9 were identified by all three. miR-Oncology database screening identified CDK17, PRDM1, and E2F3 as potential miR-3691-3p target genes closely related to the occurrence and development of tumors. (b and c) Western blot analysis (b) and RT-qPCR analysis (c) of CDK17, E2F3, and PRDM1 protein and mRNA expression, respectively, in PC-3 and DU145 cells at 48 h after transfection with a control or miR-3691-3p mimic. (d–f) DU145 and PC-3 cell lines were transfected with a control, E2F3-targeting, or PRDM1-targeting siRNA, and then analyzed. (d) Cell proliferation was assessed using the MTT assay on days one, two, and three after transfection. (e) Wound-healing migration assay performed on day one and day two after transfection. (f) Transwell invasion assay performed after transfection. Data are presented as the mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001. PCa: prostate cancer; CDK17: cyclin dependent kinase 17; RT-qPCR: real-time quantitative polymerase chain reaction; PRDM1: PR domain containing 1, with ZNF domain; MTT: methyl thiazolyl tetrazolium; OD: optical density; E2F3: E2F transcription factor 3; s.d.: standard deviation.

Figure 3: Effect of knockdown of endogenous miR-3691-3p target genes on the behavior of PCa cells. (<b>a</b>) Of the 200 target genes identified from three bioinformatic algorithms (DIANA-MICROT, Target Scan, and MIRDB), 9 were identified by all three. miR-Oncology database screening identified <i>CDK17</i>, <i>PRDM1</i>, and <i>E2F3</i> as potential miR-3691-3p target genes closely related to the occurrence and development of tumors. (<b>b and c</b>) Western blot analysis (<b>b</b>) and RT-qPCR analysis (<b>c</b>) of <i>CDK17</i>, <i>E2F3</i>, and <i>PRDM1</i> protein and mRNA expression, respectively, in PC-3 and DU145 cells at 48 h after transfection with a control or miR-3691-3p mimic. (<b>d–f</b>) DU145 and PC-3 cell lines were transfected with a control, <i>E2F3</i>-targeting, or <i>PRDM1</i>-targeting siRNA, and then analyzed. (<b>d</b>) Cell proliferation was assessed using the MTT assay on days one, two, and three after transfection. (<b>e</b>) Wound-healing migration assay performed on day one and day two after transfection. (<b>f</b>) Transwell invasion assay performed after transfection. Data are presented as the mean ± s.d. *<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001. PCa: prostate cancer; <i>CDK17</i>: cyclin dependent kinase 17; RT-qPCR: real-time quantitative polymerase chain reaction; <i>PRDM1</i>: PR domain containing 1, with ZNF domain; MTT: methyl thiazolyl tetrazolium; OD: optical density; <i>E2F3</i>: E2F transcription factor 3; s.d.: standard deviation.