Figure 2: Effect of miR-3691-3p overexpression on the behavior of PCa cell lines. DU145 and PC-3 cells were transfected with a control or miR-3691-3p mimic and then analyzed. (a) Cell proliferation was analyzed by the MTT assay on days 1, 3 and 5 after transfection. (b) Apoptosis was analyzed using an Annexin V-FITC/PI flow cytometry assay. Representative plots of DU145 cells (upper panels) and PC-3 cells (lower panels) are shown on day 2 after transfection. (c) Representative images (upper panels) and quantification (lower panels) of migration on a wound-healing assay of PCa cells on day one and ady two after transfection. (d) Transwell invasion assay of PCa cells after transfection. Invasion was quantified by counting five independent symmetrical visual fields under the microscope. Data are presented as the mean ± s.d. of one experiment, representative of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. PCa: prostate cancer; MTT: methyl thiazolyl tetrazolium; FITC: fluorescein isothiocyanate; PI: propidium iodide; OD: optical density; s.d.: standard deviation.