Figure 1: Expression levels of miR-3691-3p in PCa cell lines and tissues. (a) RT-qPCR analysis of five miRNAs in PC-3 and DU145 cells incubated with or without 5 ng ml−1 TGF-β1 for 24 h. (b) RT-qPCR analysis of five TGF-β1-regulated miRNAs in five paired samples of human PCa and BPH tissues. (c) RT-qPCR analysis of miR-3691-3p expression in 100 paired samples of PCa and normal prostate tissues. Data are presented as the mean ± s.d. of three independent experiments. (d) PCa patients were dichotomized into high and low miR-3691-3p expression groups using the median expression level as the cut-off value. Kaplan–Meier curves show BCR-free survival. Data are presented as the mean ± s.d. of one experiment, representative of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. RT-qPCR: real-time quantitative polymerase chain reaction; TGF-β1: transforming growth factor beta 1; PCa: prostate cancer; BPH: benign prostatic hyperplasia; BCR: biochemical recurrence; s.d.: standard deviation.