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  Indian J Med Microbiol
 

Figure 2: The dose-response of human spermatozoa to GTE under oxidative stress condition. The pellet of 15 normal semen parameter samples was isolated using density gradient centrifugation. The pellet was washed and resuspended in capacitated media and pooled in 5 pools (three samples each). Mixed spermatozoa were treated with GTE (0.1 ng ml-1, 1.0 ng ml-1, 10 ng ml-1, and 100 ng ml-1) before the exposure to H2O2 for 15 min. (a) Percentage of total motility. (b) Percentage of progressive motility. Data are expressed as mean ± standard deviation of five experiments using different pools. Statistical significance levels were measured by One Way RM ANOVA and indicated as: #P < 0.05 versus control (post-DGC sample); *P < 0.05 versus H2O2. GTE: green tea extract; RM: repeated measures; DGC: density gradient centrifugation.

Figure 2: The dose-response of human spermatozoa to GTE under oxidative stress condition. The pellet of 15 normal semen parameter samples was isolated using density gradient centrifugation. The pellet was washed and resuspended in capacitated media and pooled in 5 pools (three samples each). Mixed spermatozoa were treated with GTE (0.1 ng ml<sup>-1</sup>, 1.0 ng ml<sup>-1</sup>, 10 ng ml<sup>-1</sup>, and 100 ng ml<sup>-1</sup>) before the exposure to H2O2 for 15 min. (<b>a</b>) Percentage of total motility. (<b>b</b>) Percentage of progressive motility. Data are expressed as mean ± standard deviation of five experiments using different pools. Statistical significance levels were measured by One Way RM ANOVA and indicated as: <sup>#</sup><i>P</i> < 0.05 versus control (post-DGC sample); *<i>P</i> < 0.05 versus H<sub>2</sub>O<sub>2</sub>. GTE: green tea extract; RM: repeated measures; DGC: density gradient centrifugation.