Figure 4: Administration of KF reduced mast cell number in EAO. Representative microphotographs of Alcian blue staining performed on EAO testis sections. Mast cells are observed near the tunica albuginea and vessels of both, (a and b)untreated or (c and d)treated with KF rats. Scale bars = 50 μm in a and c; and 20 μm in b and d. (e) Quantitative assessment of Alcian blue-positive mast cell number in EAO testis sections from rats treated with KF (treatment 1) or saline solution (vehicle). Horizontal lines represent the mean value and each symbol represents a single rat. P = 0.020 (Mann–Whitney U test). EAO: experimental autoimmune orchitis; ST: seminiferous tubule; KF: ketotifen fumarate.