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  Indian J Med Microbiol
 

Figure 2: Relative luciferase activities of pmir-rs1052482A and pmir-rs1052482T in HEK293T cells. a - c represent the mean RLU values ± s.d. of triplicates. ( a ) No significant differences in relative luciferase activity among three vectors. ( b ) Luciferase activity was substantially decreased in cells cotransfected with pmir-rs1052482A and miR-3162-5p compared to the cells cotransfected with pmir-control and miR-3162-5p (P < 0.01). ( c ) A sequential decrease was observed in cells cotransfected with pmir-rs1052482A and miR-3162-5p in proportion to the amount of the mimic and this reduction was rescued when the mir-3162-5p inhibitor was cotransfected with pmir-rs1052482A and miR-3162-5p. -: absence of the indicated one; +: presence of the indicated one; *P < 0.05 (pmir-rs1052482A + mir3162-5p [5 pmol] compared to pmir-rs1052482A); **P < 0.01 (pmir-rs1052482A + mir3162-5p [10 pmol] compared to pmir-rs1052482A). CTL: control; RLU: relative light unit; s.d.: standard deviation.

Figure 2: Relative luciferase activities of pmir-rs1052482A and pmir-rs1052482T in HEK293T cells. a - c represent the mean RLU values ± s.d. of triplicates. ( a ) No significant differences in relative luciferase activity among three vectors. ( b ) Luciferase activity was substantially decreased in cells cotransfected with pmir-rs1052482A and <i>miR-3162-5p</i> compared to the cells cotransfected with pmir-control and <i>miR-3162-5p</i> (<i>P</i> < 0.01). ( c ) A sequential decrease was observed in cells cotransfected with pmir-rs1052482A and <i>miR-3162-5p</i> in proportion to the amount of the mimic and this reduction was rescued when the <i>mir-3162-5p</i> inhibitor was cotransfected with pmir-rs1052482A and <i>miR-3162-5p.</i> -: absence of the indicated one; +: presence of the indicated one; *<i>P <</i> 0.05 (pmir-rs1052482A + mir3162-5p [5 pmol] compared to pmir-rs1052482A); **<i>P</i> < 0.01 (pmir-rs1052482A + mir3162-5p [10 pmol] compared to pmir-rs1052482A). CTL: control; RLU: relative light unit; s.d.: standard deviation.