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  Indian J Med Microbiol
 

Figure 6: Asymmetrically divided Apr clones, false Aun and Aun–Adiff clones were identified by co-staining TEX14 with GFRA1 and PLZF. (a) GFRA1 asymmetrically positive Apr clones in whole-mount IHC were identified as asymmetrical division by the presence of TEX14. (b) GFRA1 asymmetrically stained Aun clones in whole-mount IHC was identified as false Aun clone by the absence of TEX14 between adjacent GFRA1POS and GFRA1NEG clonal members. (c) a PLZF asymmetrically expressed Aun–Adiff clone in whole-mount IHC was identified as false Aun and Aun–Adiff clone by absence of TEX14 between adjacent Aun and Adiff clonal members. GFRA1POS or PLZFHIGH Aun: red arrows; GFRA1NEG Aun: yellow arrows; PLZFLOW Adiff : green arrows; IB in whole mount IHC: white arrowheads; IB between Aun connected by TEX14 in whole mount IF: white arrowheads; IB between Adiff connected by TEX14 in whole mount IF: green arrowhead; IB between asymmetrical GFRA1 or PLZF cells without TEX14 in whole mount IF– yellow arrowhead. IB: intercellular bridge. Scale bar=25 μm.

Figure 6: Asymmetrically divided A<sub>pr</sub> clones, false A<sub>un</sub> and A<sub>un</sub>–A<sub>diff</sub> clones were identified by co-staining TEX14 with GFRA1 and PLZF. (<b>a</b>) GFRA1 asymmetrically positive A<sub>pr</sub> clones in whole-mount IHC were identified as asymmetrical division by the presence of TEX14. (<b>b</b>) GFRA1 asymmetrically stained A<sub>un</sub> clones in whole-mount IHC was identified as false A<sub>un</sub> clone by the absence of TEX14 between adjacent GFRA1<sup>POS</sup> and GFRA1<sup>NEG</sup> clonal members. (<b>c</b>) a PLZF asymmetrically expressed A<sub>un</sub>–A<sub>diff</sub> clone in whole-mount IHC was identified as false A<sub>un</sub> and A<sub>un</sub>–A<sub>diff</sub> clone by absence of TEX14 between adjacent A<sub>un</sub> and A<sub>diff</sub> clonal members. GFRA1<sup>POS</sup> or PLZF<sup>HIGH</sup> A<sub>un</sub>: red arrows; GFRA1<sup>NEG</sup> A<sub>un</sub>: yellow arrows; PLZF<sup>LOW</sup> A<sub>diff</sub> : green arrows; IB in whole mount IHC: white arrowheads; IB between A<sub>un</sub> connected by TEX14 in whole mount IF: white arrowheads; IB between A<sub>diff</sub> connected by TEX14 in whole mount IF: green arrowhead; IB between asymmetrical GFRA1 or PLZF cells without TEX14 in whole mount IF– yellow arrowhead. IB: intercellular bridge. Scale bar=25 μm.