Figure 2: Morphological characterization and flow cytometry analysis. (a) Morphological characterization by fluorescence microscopy: LC after immunostaining with AMH antibody and visualized by anti-goat Alexa Fluor 488 (green). (b) LC after immunostaining with 3β-HSD antibody and visualized by anti-goat Alexa Fluor 488 (green). (c) LC after immunostaining with ASMA antibody and visualized by anti-rabbit Alexa Fluor 488 (green). Nuclei are counterstained with DAPI (blue). In each figure, the magnification insert is showed in the upper right and the negative controls' insert is displayed in the bottom left. Flow cytometry analysis of cultured LC for (d) AMH, (e) 3β-HSD, and (f) ASMA. The images are representative of three separate experiments. LC: Leydig cells; AMH: anti-Müllerian hormone; ASMA: alpha-smooth muscle actin; 3β-HSD: 3β-hydroxysteroid dehydrogenase.