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Figure 6: mRNA expression of apoptosis-related genes in gonocytes treated with or without retinoic acid (RA). mRNA expression of apoptosis-related genes was measured by quantitative polymerase chain reaction analysis of gonocytes treated with or without RA (10−6 M). Stra8 mRNA levels were also measured as an internal control for differentiation. Results are fold change values overexpression levels in untreated gonocytes and are expressed as means ± SEM of data obtained from 3 to 5 independent cell preparations, each sample performed in triplicate (*P < 0.05, **P < 0.001). |
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