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  Indian J Med Microbiol
 

Figure 1: Patient with CBAVD and NOA. (a) Hematoxylin–eosin staining of testicular tissue sections shows normal spermatogenesis. Scale bars = 50 μm (left) and 20 μm (right), respectively. Red arrow: Sertoli cells; blue arrow: germ cells. (b) The diameter of the seminiferous tubules of the patient was significantly smaller than that in the normal male. **P < 0.01. (c) Immunofluorescence staining shows spermatogenesis in testicular tissue sections representing normal spermatogenesis and SCOS. Staining for CFTR (green) is weak, and staining for DDX4 (red) is absent, in testicular tubules from the proband (blue: DAPI; scale bars = 50 μm). (d) Immunofluorescence staining shows spermatogenesis in normal and SCOS afflicted testicular tissue sections. Weak CFTR (green) and SOX9 (red) staining indicates Sertoli cells in testicular tubules from the proband (blue: DAPI; scale bars = 50 μm). SCOS: Sertoli cell-only syndrome; DDX4: DEAD-box helicase 4; SOX9: SRY-box transcription factor 9; DAPI: 4',6-diamidino-2-phenylindole; CFTR: cystic fibrosis transmembrane conductance regulator; CBAVD: congenital bilateral absence of vas deferens; NOA: nonobstructive azoospermia.

Figure 1: Patient with CBAVD and NOA. (<b>a</b>) Hematoxylin–eosin staining of testicular tissue sections shows normal spermatogenesis. Scale bars = 50 μm (left) and 20 μm (right), respectively. Red arrow: Sertoli cells; blue arrow: germ cells. (<b>b</b>) The diameter of the seminiferous tubules of the patient was significantly smaller than that in the normal male. <sup>**</sup><i>P</i> < 0.01. (<b>c</b>) Immunofluorescence staining shows spermatogenesis in testicular tissue sections representing normal spermatogenesis and SCOS. Staining for CFTR (green) is weak, and staining for DDX4 (red) is absent, in testicular tubules from the proband (blue: DAPI; scale bars = 50 μm). (<b>d</b>) Immunofluorescence staining shows spermatogenesis in normal and SCOS afflicted testicular tissue sections. Weak CFTR (green) and SOX9 (red) staining indicates Sertoli cells in testicular tubules from the proband (blue: DAPI; scale bars = 50 μm). SCOS: Sertoli cell-only syndrome; DDX4: DEAD-box helicase 4; SOX9: SRY-box transcription factor 9; DAPI: 4',6-diamidino-2-phenylindole; CFTR: cystic fibrosis transmembrane conductance regulator; CBAVD: congenital bilateral absence of vas deferens; NOA: nonobstructive azoospermia.