Asian Journal of Andrology

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 18  |  Issue : 5  |  Page : 735--746

Proteomic analysis of mature and immature ejaculated spermatozoa from fertile men


Zhihong Cui, Rakesh Sharma, Ashok Agarwal 
 American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USA; Institute of Toxicology, Third Military Medical University, Chongqing 400038, PR China

Correspondence Address:
Ashok Agarwal
American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195
USA

Dysfunctional spermatozoa maturation is the main reason for the decrease in sperm motility and morphology in infertile men. Ejaculated spermatozoa from healthy fertile men were separated into four fractions using three-layer density gradient. Proteins were extracted and bands were digested on a LTQ-Orbitrap Elite hybrid mass spectrometer system. Functional annotations of proteins were obtained using bioinformatics tools and pathway databases. Western blotting was performed to verify the expression levels of the proteins of interest. 1469 proteins were identified in four fractions of spermatozoa. The number of detected proteins decreased according to the maturation level of spermatozoa. During spermatozoa maturation, proteins involved in gamete generation, cell motility, energy metabolism and oxidative phosphorylation processes showed increasing expression levels and those involved in protein biosynthesis, protein transport, protein ubiquitination, and response to oxidative stress processes showed decreasing expression levels. We validated four proteins (HSP 70 1A, clusterin, tektin 2 and tektin 3) by Western blotting. The study shows protein markers that may provide insight into the ejaculated spermatozoa proteins in different stages of sperm maturation that may be altered or modified in infertile men.


How to cite this article:
Cui Z, Sharma R, Agarwal A. Proteomic analysis of mature and immature ejaculated spermatozoa from fertile men.Asian J Androl 2016;18:735-746


How to cite this URL:
Cui Z, Sharma R, Agarwal A. Proteomic analysis of mature and immature ejaculated spermatozoa from fertile men. Asian J Androl [serial online] 2016 [cited 2022 Oct 4 ];18:735-746
Available from: https://www.ajandrology.com/article.asp?issn=1008-682X;year=2016;volume=18;issue=5;spage=735;epage=746;aulast=Cui;type=0