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Update on techniques for cryopreservation of human spermatozoa


1 Institute of Reproductive and Stem Cell Engineering, Basic Medicine College, Central South University, Changsha 410000, China
2 Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha 410000, China
3 Department of Andrology, Center for Men's Health, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai 200025, China

Correspondence Address:
Wen-Bing Zhu,
Institute of Reproductive and Stem Cell Engineering, Basic Medicine College, Central South University; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha 410000
China
Zheng Li,
Department of Andrology, Center for Men's Health, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai 200025
China
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/aja20229

PMID: 35381695

In the 1960s, sperm cryopreservation was developed as a method to preserve fertility. Currently, techniques for the cryopreservation of human spermatozoa have been widely used in assisted reproduction. However, although sperm cryobiology has made notable achievements, the optimal method for the recovery of viable spermatozoa after cryopreservation remains elusive. Postthawing sperm quality can be affected by cryoprotectants, ice formation, storage conditions, and osmotic stress during the freezing process. This review discusses recent advances in different cryopreservation techniques, cryoprotectants, and freezing and thawing methods during cryopreservation and new indications for the use of cryopreserved spermatozoa.


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