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A novel mouse model simulating transurethral laser vaporization prostatectomy


1 Medical School of Guizhou University, Guiyang 550000, China
2 Department of Urology Surgery, Guizhou Province People's Hospital, Guiyang 550002, China
3 School of Clinical Medicine, Guizhou Medical University, Guiyang 55025, China
4 School of Clinical Medicine, Guangzhou Medical University, Guangzhou 510180, China
5 Department of Urology, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai 201620, China

Correspondence Address:
Guang-Heng Luo,
Medical School of Guizhou University, Guiyang 550000; Department of Urology Surgery, Guizhou Province People's Hospital, Guiyang 550002
China
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/aja.aja_59_21

PMID: 34259200

Benign prostatic hyperplasia (BPH) is a common disease in elderly men, and transurethral laser prostatectomy (TULP) has been widely used in the clinic to remove bladder outlet obstruction caused by BPH. Previous animal models for wound repair after prostatectomy have many limitations, and there have been no previous reports of a mouse model of TULP. Therefore, this study aimed to establish a novel mouse model of TULP. Twelve healthy adult Kunming (KM) mice received transurethral laser vaporization prostatectomy with a 200-μm thulium laser. The mice were sacrificed, and wound specimens from the prostatic urethra and bladder neck were harvested at 1 day, 3 days, 5 days, and 7 days after surgery. Hematoxylin-eosin (HE) and immunohistochemistry were applied to confirm the establishment of the mouse TULP model. One day after the surgery, urothelium expressing uroplakin (UPK) was absent in the urethral wound site, and a large number of necrotic tissues were found in the wound site. There was no UPK-positive urothelium in the wound 3 days after surgery. At 5 days after surgery, monolayer urothelium expressing UPK was found in the wound site, indicating that the re-epithelization of the wound had been completed. On the 7th day after surgery, there were multiple layers of urothelium with UPK expression, indicating that the repair was completed. It is feasible to establish a mouse TULP model by using a microcystoscope system and a 200-μm thulium laser.


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