REVIEW
Year : 2022  |  Volume : 24  |  Issue : 6  |  Page : 563-569

Update on techniques for cryopreservation of human spermatozoa


1 Institute of Reproductive and Stem Cell Engineering, Basic Medicine College, Central South University, Changsha 410000, China Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha 410000, China Department of Andrology, Center for Men's Health, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai 200025, China

Correspondence Address:
Wen-Bing Zhu
Institute of Reproductive and Stem Cell Engineering, Basic Medicine College, Central South University; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha 410000
China
Zheng Li
Department of Andrology, Center for Men's Health, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai 200025
China
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/aja20229

Rights and Permissions

In the 1960s, sperm cryopreservation was developed as a method to preserve fertility. Currently, techniques for the cryopreservation of human spermatozoa have been widely used in assisted reproduction. However, although sperm cryobiology has made notable achievements, the optimal method for the recovery of viable spermatozoa after cryopreservation remains elusive. Postthawing sperm quality can be affected by cryoprotectants, ice formation, storage conditions, and osmotic stress during the freezing process. This review discusses recent advances in different cryopreservation techniques, cryoprotectants, and freezing and thawing methods during cryopreservation and new indications for the use of cryopreserved spermatozoa.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2450    
    Printed160    
    Emailed0    
    PDF Downloaded234    
    Comments [Add]    

Recommend this journal