Table of Contents  
Year : 2021  |  Volume : 23  |  Issue : 6  |  Page : 600-601

The use of spermatogonial stem cells to correct a mutation causing meiotic arrest

Center for Reproductive Medicine, Reproductive Biology Laboratory, Amsterdam Reproduction and Development Research Institute, Amsterdam UMC, Location AMC, University of Amsterdam, Meibergdreef 9, Amsterdam 1105 AZ, The Netherlands

Date of Submission07-Dec-2020
Date of Acceptance20-Dec-2020
Date of Web Publication05-Feb-2021

Correspondence Address:
Geert Hamer
Center for Reproductive Medicine, Reproductive Biology Laboratory, Amsterdam Reproduction and Development Research Institute, Amsterdam UMC, Location AMC, University of Amsterdam, Meibergdreef 9, Amsterdam 1105 AZ
The Netherlands
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/aja.aja_2_21

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How to cite this article:
Lei Q, Hamer G. The use of spermatogonial stem cells to correct a mutation causing meiotic arrest. Asian J Androl 2021;23:600-1

How to cite this URL:
Lei Q, Hamer G. The use of spermatogonial stem cells to correct a mutation causing meiotic arrest. Asian J Androl [serial online] 2021 [cited 2021 Dec 9];23:600-1. Available from: - DOI: 10.4103/aja.aja_2_21

The basis of life-long spermatogenesis is spermatogonial stem cells (SSCs) located at the basal membrane of the seminiferous tubules in the testis. After several rounds of proliferation and spermatogonial differentiation, the male germ cells will eventually undergo meiosis to form haploid spermatids. Disturbance of the molecular regulation of spermatogenesis can lead to spermatogenic arrest, in humans often during meiosis,[1],[2] and subsequent azoospermia. Unfortunately, no treatment option enabling conception of a genetically own child is currently available for men suffering from spermatogenic arrest before spermatids are formed.

In this issue of the Asian Journal of Andrology, Wang et al.[3] present the use of cultured SSCs to restore a mutation, which otherwise leads to meiotic arrest, by clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated endonuclease 9 (CRISPR-Cas9)-mediated genome editing. This would theoretically open the possibility to use a patient's own SSCs to correct the mutation that causes meiotic arrest, followed by an autotransplantation to the patient's testes where the corrected SSCs would be able to initiate complete spermatogenesis.[4]

Besides mutations causing meiotic arrest, potentially any mutation that can cause a severe monogenetic disease in the offspring can be corrected in cultured SSCs. Indeed, in 2015, Wu et al.[5] were able to correct a mutation in the gene crystallin gamma C (Crygc; which leads to blindness when uncorrected) in cultured SSCs. Upon transplantation to testes that lack endogenous spermatogenesis, these restored SSCs were able to find the stem cell niche and generate wild-type spermatids. These spermatids were used to generate offspring without the blindness-causing mutation.[5]

One problem that remains is that, in most clinical settings, the recipient testes still contain endogenous germ cells. These endogenous germ cells still harbor the mutation. Therefore, even after autotransplantation of corrected SSCs, offspring may still be fathered by sperm derived from uncorrected endogenous SSCs. These endogenous SSCs should thus be removed from the patient's testes before autotransplantation of the corrected SSCs. Because this would require irradiation, or genotoxic chemicals such as busulfan, clinical application of autotransplantation of corrected SSCs is currently not feasible.[4]

One exception may be correction of mutations that cause meiotic arrest. In these cases, no endogenous spermatids are formed, so all offspring will be derived from germ cells with the corrected gene. Wang et al.[3] have now generated mice that harbor a mutation in the gene testis expressed 11 (Tex11) that mimics a similar mutation that causes meiotic arrest in humans. As published for the Crygc gene,[5] CRISPR-Cas9 was applied to correct the Tex11 mutation followed by transplantation to recipient testes lacking endogenous spermatogenesis. As expected, this enabled wild-type spermatogenesis in the recipient testes and generation of fertile offspring.

However, typically, a human male harboring a mutation in the TEX11 gene, or any patient suffering from meiotic arrest, still has his endogenous germ cells up until the spermatocyte stage before the arrest. Although not generating sperm, these endogenous germ cells may still hinder homing of the autotransplanted SSCs to the basal membrane of the seminiferous tubules. The question thus is whether autotransplanted corrected SSCs will reach the basal membrane of the seminiferous tubules, from where they can start to grow and differentiate, when the patient's endogenous germ cells potentially block the way. To answer this question, Wang et al.[3] also transplanted corrected spermatogonia to Tex11- mutated testes that contain germ cells up until the arrested spermatocyte stage. Importantly, in this case, they did not find any corrected SSCs to reach the basal membrane and no complete spermatogenesis was observed after the SSC transplantation. Hence, the endogenous germ cells hindered homing, and thus subsequent spermatogenesis, of the corrected SSCs.

For further development of this method for possible clinical application, this is a crucial finding. Removal of the endogenous germ cells from the patient's testes by irradiation or genotoxic chemicals does not seem to be a healthy solution at this moment. Perhaps it is not the germ cells themselves but an intact blood–testis barrier that hinders homing of the corrected SSCs. The blood–testis barrier consists of Sertoli–germ cell junctions, as well as Sertoli–Sertoli cell junctions that divide the seminiferous epithelium into a basal and adluminal compartment.[6] Perhaps, development of a way to temporally disturb this barrier would enable homing of the corrected SSCs. Another option may be use of the genetically corrected SSCs to generate sperm in vitro, thereby omitting the need for transplantation.[7],[8] However, the methods to achieve this have not been sufficiently developed. For instance, the meiotic checkpoints that are normally active during meiosis in vivo to prevent genomic aberrations to be transmitted to the offspring are not necessarily also active in vitro.[8]

Hence, Wang et al.[3] have perfectly illustrated the possibilities and problems of using autotransplantation of genetically corrected male germline stem cells to “restore” spermatogenesis in men suffering from meiotic arrest. To our knowledge, their paper is the first to demonstrate that endogenous germ cells can block homing of transplanted SSCs. Although this may feel as a setback, this piece of information is crucial for further consideration and development of a treatment option for male meiotic arrest patients that is based on autotransplantation of SSCs.

  Competing Interests Top

Both authors declare no competing interests.

  References Top

Jan SZ, Jongejan A, Korver CM, van Daalen SK, van Pelt AM, et al. Distinct prophase arrest mechanisms in human male meiosis. Development 2018; 145: dev160614.  Back to cited text no. 1
Enguita-Marruedo A, Sleddens-Linkels E, Ooms M, de Geus V, Wilke M, et al. Meiotic arrest occurs most frequently at metaphase and is often incomplete in azoospermic men. Fertil Steril 2019; 112: 1059–70.e3.  Back to cited text no. 2
Wang YH, Yan M, Zhang X, Liu XY, Ding YF, et al. Rescue of male infertility through correcting a genetic mutation causing meiotic arrest in spermatogonial stem cells. Asian J Androl 2021. Doi: 10.4103/aja.aja_97_20. [Epub ahead of print].  Back to cited text no. 3
Mulder CL, Zheng Y, Jan SZ, Struijk RB, Repping S, et al. Spermatogonial stem cell autotransplantation and germline genomic editing: a future cure for spermatogenic failure and prevention of transmission of genomic diseases. Hum Reprod Update 2016; 22: 561–73.  Back to cited text no. 4
Wu Y, Zhou H, Fan X, Zhang Y, Zhang M, et al. Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells. Cell Res 2015; 25: 67–79.  Back to cited text no. 5
Mruk DD, Cheng CY. The mammalian blood-testis barrier: its biology and regulation. Endocr Rev 2015; 36: 564–91.  Back to cited text no. 6
Sun M, Yuan Q, Niu M, Wang H, Wen L, et al. Efficient generation of functional haploid spermatids from human germline stem cells by three-dimensional-induced system. Cell Death Differ 2018; 25: 749–66.  Back to cited text no. 7
Lei Q, Lai X, Eliveld J, de Sousa Lopes SM, van Pelt AM, et al. In vitro meiosis of male germline stem cells. Stem Cell Reports 2020; 15: 1140–53.  Back to cited text no. 8


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